期刊
TRANSGENIC RESEARCH
卷 26, 期 1, 页码 13-23出版社
SPRINGER
DOI: 10.1007/s11248-016-9977-x
关键词
Rice (Oryza sativa L.); Astaxanthin; Ketocarotenoids; Carotenoids; beta-carotene ketolase; Multigene transformation
类别
资金
- Ministerio de Economia y Competitividad, Spain [BIO2014-54441-P, BIO2014-54426-P, BIO2011-23324, BIO2011-22525, PIM2010PKB-00746]
- Plan Nacional de I + D + I
- IBERCAROT network [112RT0445]
- PROGRAMA ESTATAL DE INVESTIGACION CIENTIFICA Y TECNICA DE EXCELENCIA, Spain [BIO2015-71703-REDT]
- COST action (EUROCAROTEN) [OC-2015-1-19780]
- European Union European Research Council IDEAS Advanced Grant
- Program-BIOFORCE
- ERC Proof of Concept Grant
- Catalan Government Agricultural Biotechnology Research Group [SGR 1296]
- Universitat de Lleida, Spain
- ICREA Funding Source: Custom
Astaxanthin is a high-value ketocarotenoid rarely found in plants. It is derived from beta-carotene by the 3-hydroxylation and 4-ketolation of both ionone end groups, in reactions catalyzed by beta-carotene hydroxylase and beta-carotene ketolase, respectively. We investigated the feasibility of introducing an extended carotenoid biosynthesis pathway into rice endosperm to achieve the production of astaxanthin. This allowed us to identify potential metabolic bottlenecks that have thus far prevented the accumulation of this valuable compound in storage tissues such as cereal grains. Rice endosperm does not usually accumulate carotenoids because phytoene synthase, the enzyme responsible for the first committed step in the pathway, is not present in this tissue. We therefore expressed maize phytoene synthase 1 (ZmPSY1), Pantoea ananatis phytoene desaturase (PaCRTI) and a synthetic Chlamydomonas reinhardtii beta-carotene ketolase (sCrBKT) in transgenic rice plants under the control of endosperm-specific promoters. The resulting grains predominantly accumulated the diketocarotenoids canthaxanthin, adonirubin and astaxanthin as well as low levels of monoketocarotenoids. The predominance of canthaxanthin and adonirubin indicated the presence of a hydroxylation bottleneck in the ketocarotenoid pathway. This final rate-limiting step must therefore be overcome to maximize the accumulation of astaxanthin, the end product of the pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据