期刊
TRAFFIC
卷 17, 期 5, 页码 487-499出版社
WILEY-BLACKWELL
DOI: 10.1111/tra.12386
关键词
actin; dynamin; phagocytosis; pseudopods; macrophages; scission
类别
资金
- CNRS
- Inserm
- Universite Paris Descartes
- Agence Nationale de la Recherche [2011 BSV3 025 02]
- Fondation pour la Recherche Medicale ('Equipe FRM') [FRM DEQ20130326518]
- Agence Nationale de la Recherche sur le SIDA et les Hepatites (ANRS)
Phagocytosis is a mechanism used by macrophages to internalize and eliminate microorganisms or cellular debris. It relies on profound rearrangements of the actin cytoskeleton that is the driving force allowing plasma membrane extension around the particle. The closure step of phagocytosis, however, remains poorly defined. We used a dedicated experimental setup with Total Internal Reflection Fluorescence Microscopy (TIRFM) to monitor phagosome formation and closure in three dimensions in living cells. We show that dynamin-2, which mediates the scission of endocytic vesicles, was recruited early and concomitantly with actin during phagosome formation. Dynamin-2 accumulated at the site of phagosome closure in living macrophages. Inhibition of its activity with dominant negative mutants or drugs demonstrated that dynamin-2 is implicated in actin dynamics and pseudopod extension. Depolymerization of actin led to impaired dynamin-2 recruitment or activity. Finally, we show that dynamin-2 plays a critical role in the effective scission of the phagosome from the plasma membrane. Thus, we establish that a cross talk between actin and dynamin takes place for phagosome formation and closure before dynamin functions for scission.
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