期刊
TOXICOLOGY LETTERS
卷 242, 期 -, 页码 23-33出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2015.11.025
关键词
Cytokine; Dendritic cell; Migration; Piperine; T lymphocyte
类别
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- NSERC
- NSHRF
- Canadian Institutes of Health Research/Crohn's and Colitis Foundation of Canada/Nova Scotia Health Research Foundation (NSHRF)
Piperine, a major alkaloid found in the fruits of black and long pepper plants, has anti-inflammatory properties; however, piperine's effect on dendritic cell (DC) migration and T cell-activating function has not been investigated. Bone marrow-derived mouse DCs that were matured in the presence of 100 mu M piperine showed reduced in vitro migration in response to CCL21, as well as reduced in vivo migration to lymph nodes. In addition, piperine-treated DCs had reduced CCR7 expression and elevated CCR5 expression, as well as reduced expression of CD40 and class II major histocompatibility complex molecules and decreased nuclear accumulation of RelB. DC production of interleukin (IL)-6, tumor necrosis factor alpha, and monocyte chemoattractant protein-1 in response to lipopolysaccharide stimulation was also reduced following piperine treatment. Exposure to piperine during maturation therefore caused DCs to retain an immature phenotype, which was associated with a reduced capacity to promote T cell activation since co-culture of ovalbumin (OVA(323-339))-specific T cells with OVA(323-339)-pulsed DCs that were previously matured in the presence of piperine showed reduced interferon-gamma and IL-2 expression. OVA(323-339)-specific T cell proliferation was also reduced in vivo in the presence of piperine-treated DCs. Inhibition of DC migration and function by piperine may therefore be a useful strategy to down-regulate potentially harmful DC-driven T cell responses to self-antigens and transplantation antigens. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据