期刊
TOXICOLOGY AND INDUSTRIAL HEALTH
卷 32, 期 11, 页码 1848-1858出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/0748233715590758
关键词
Bisphenol A; estrogen to androgen ratio; fluorescent real-time PCR; genome oligo microarray analysis; in vivo; low dose; proliferation; prostaglandin D-2 synthase; prostate; rat
资金
- National Natural Science Foundation of China [21007041]
- Shanghai Public Service Platform of Research Development [13DZ2291300]
- Talents Development Foundation of Shanghai Municipality [201372]
This study aims to assess the effect of low oral dose of bisphenol A (BPA) on proliferation of ventral prostate (VP) and expression of related genes in adult rats. Three-month-old male Sprague Dawley rats were treated daily with BPA (10, 30, or 90 mu g/kg, per os), 17 beta-estradiol (E-2, 10.0 mu g/kg, subcutaneously), or vehicle for 4 weeks. Treatment with 10,g/kg BPA resulted in increased animal weight and VP epithelial height compared with the controls (p < 0.01), while such effects were less pronounced in higher BPA doses. Treatment with E2 showed opposite effects, with significantly decreased animal weight and VP epithelial height (p < 0.01). Interestingly, BPA increased serum E-2 and reduced testosterone levels and significantly increased the estrogen to androgen ratio (p < 0.05). In addition, BPA slightly increased dihydrotestosterone (DHT) levels. Immunohistochemistry data showed that BPA significantly upregulated proliferating cell nuclear antigen expression (p < 0.01). Furthermore, microarray and reverse transcription polymerase chain reaction analyses showed that BPA induced upregulation of prostaglandin D-2 synthase (Ptgds), Fas, Pbefl, and complement factor B (Cfb)as well as downregulation of Pttgl and Fabp4 in the VP. These results indicated that environmental exposure to low doses of BPA may induce proliferation of VP in adult rats by increasing the estrogen to androgen ratio and upregulating expression of Ptgds to promote production of DHT.
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