期刊
TOXICOLOGY AND APPLIED PHARMACOLOGY
卷 303, 期 -, 页码 65-78出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.taap.2016.04.016
关键词
Ovary; Chromium; Resveratrol; Antioxidants; Oxidative Stress; Steroidogenesis
资金
- National Institute of Environmental Health Sciences (NIEHS) [ES020561-01, ES025234-01A1]
- Center for Translational Environmental Health Research (CTEHR) [P30ES023512]
- NIH-NCRR Shared Instrumentation Grant [1 S10 RR22532-01]
Resveratrol (RVT), a polyphenolic component in grapes and red wine, has been known for its cytoprotective actions against several diseases. However, beneficial effects of RVT against early exposure to endocrine disrupting chemicals (EDCs) have not been understood. EDCs are linked to several ovarian diseases such as premature ovarian failure, polycystic ovary syndrome, early menopause and infertility in women. Hexavalent chromium (CrVI) is a heavy metal EDC, and widely used in >50 industries. Environmental contamination with CrVI in the US is rapidly increasing, predisposing the human to several illnesses including cancers and still birth. Our lab has been involved in determining the molecular mechanism of CrVI-induced female infertility and intervention strategies to mitigate CrVI effects. Lactating mother rats were exposed to CrVI (50 ppm potassium dichromate) from postpartum days 1-21 through drinking water with or without RVT (10 mg/kg body wt., through oral gavage daily). During this time, F1 females received respective treatments through mother's milk. On postnatal day (PND) 25, blood and the ovary, kidney and liver were collected from the F1 females for analyses. CrVI increased atresia of follicles by increasing cytochrome C and cleaved caspase-3; decreasing antiapoptotic proteins; decreasing estradiol (E-2) biosynthesis and enhancing metabolic clearance of E-2, increasing oxidative stress and decreasing endogenous antioxidants. RVT mitigated the effects of CrVI by upregulating cell survival proteins and AOXs; and restored E-2 levels by inhibiting hydroxylation, glucuronidation and sulphation of E-2. This is the first study to report the protective effects of RVT against any toxicant in the ovary. (C) 2016 Published by Elsevier Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据