期刊
TISSUE ENGINEERING PART A
卷 22, 期 11-12, 页码 899-906出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/ten.tea.2015.0513
关键词
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资金
- National Basic Research Program of China [2011CB606201]
- National Natural Science Foundation of China [31100684]
- National Key Technology Support Program of China [2013BAI42G00]
- Guangxi Scientific Research and Technological Development Foundation [Guikehe 14125008-2-14]
Satisfactory repair of damaged articular cartilage is still a challenge, while tissue engineering provides a promising strategy. Collagen-based hydrogels have been widely applied in cartilage tissue engineering due to their biocompatibility. In this study, type I collagen and type II collagen were selected to prepare physically crosslinked composite hydrogels by self-assembly of collagen, and the effects of their physicochemical properties on chondrocyte phenotype maintenance and extracellular matrix (ECM) secretion were investigated. First, the microstructure of hydrogelswas observed by a scanning electron microscope, and the compressivemoduluswasmeasured by a dynamic mechanical analyzer. Then, chondrocytes were encapsulated in hydrogels and detected by Live/Dead staining. The secretion of ECM was qualitatively estimated by histological staining and quantitatively analyzed by sulfated glycosaminoglycans and DNA content detection. Finally, cartilage-specific gene expression was analyzed by quantitative real-time polymerase chain reaction analysis. The results showed that the microstructure and mechanical property of hydrogels were relevant to the composition of composite hydrogels. The compressive modulus of hydrogels improved with the increase of type I collagen content in the hydrogels. Chondrocytes could maintain their round or oval morphology and secrete cartilage-specific ECM in the four groups of hydrogels, but higher the compressive modulus of composite hydrogels, the more ECM secretion of chondrocytes.
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