期刊
THERIOGENOLOGY
卷 86, 期 5, 页码 1382-1392出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2016.04.082
关键词
Sperm; Cryopreservation extender; Stallion; Mitochondria
资金
- Center for Equine Health at UC Davis
- American Quarter Horse Association
Mitochondrial oxygen consumption is a sensitive indicator of spermatozoal health in the context of cryopreservation. We investigated oxygen consumption of equine sperm mitochondria during incubation in four commercially available sperm cryopreservation extenders: modified INRA 96, BotuCrio, EZ Freezin-LE and MFR5, in addition to several other parameters including motility, reactive oxygen species (ROS) production and viability. All experimental endpoints, with the exception of average path velocity, were affected significantly by freezing extender type after freezing and thawing. Sperm in INRA 96 had the lowest average progressive motility after thawing (24 +/- 4.8%, P < 0.05). Sperm in EZ Freezin-LE had the highest post thaw viability (79 +/- 3.1%, P < 0.05) and lowest post thaw ROS production (13 +/- 2.4%), but sperm in BotuCrio had the highest maximal oxygen consumption levels, while also demonstrating similar ROS production and viability. This difference would not have been detected using conventional sperm analytical methods. In addition, sperm in BotuCrio had the highest average total motility (49 +/- 7.4%), progressive motility (41 +/- 6.4%), and velocity (VAP, 90 +/- 3.6 mu m/s) indicating that this medium preserved mitochondrial function optimally after cryopreservation. Mitochondrial oxygen consumption was positively correlated with traditional measures of sperm function including motility and viability (r = 0.62 and r = 0.49, respectively, P < 0.05), thus making it a sensitive method for determining cryopreservation success and mitochondrial function in stallion sperm. (C) 2016 Elsevier Inc. All rights reserved.
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