期刊
TALANTA
卷 159, 期 -, 页码 1-6出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2016.05.075
关键词
MOF; Phosphopeptide enrichment; Lanthanide; Mass spectrometry
资金
- National Basic Research Priorities Program [20120910602, 20130911201]
- National Natural Science Foundation of China [21425518, 21075022, 21275033, 21105016]
- Research Fund for the Doctoral Program of Higher Education of China [20110071110007, 20100071120053]
Highly efficient enrichment of phosphopeptides from complex biological samples is crucial prior to mass spectrometry analysis due to the low abundance and ion suppression effects. In this study, a facile route was designed for preparation of a magnetic erbium(Er)-based metal-organic framework (denoted as Fe3O4@PDA@Er(btc)), which was synthesized with 1,3,5-benzenetricarboxylic acid(H(3)btc) as ligand and grafted on the polydopamine (PDA) - coated Fe3O4. The as-prepared material exhibited ultra-high sensitivity (detection limit of 20 amol/mu L) and selectivity at a low mass ratio of beta-Casein/BSA (1:500). Moreover, it was also investigated for enrichment of phosphopeptides from human serum, which provided a promising technique for highly efficient enrichment of low-abundance phosphorylated peptides in the practical application. (C) 2016 Elsevier B.V. All rights reserved.
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