期刊
TALANTA
卷 153, 期 -, 页码 211-220出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2016.03.005
关键词
Boronate affinity; Magnetic separation; Glycoprotein; Synergistic selectivity; HRP; MIP of protein
资金
- National Natural Science Foundation of China [21305107]
- Fundamental Research Funds for the Central Universities [xjj2013041, 08142034]
- China Postdoctoral Science Foundation [2014M562388]
In light of the significance of glycoprotein biomarkers for early clinical diagnostics and treatments of diseases, it is essential to develop efficient and selective enrichment platforms for glycoproteins. In this study, we present a facile and general strategy to prepare the boronate affinity-based magnetic imprinted nanoparticles. Boronic acid ligands were first grafted on the directly aldehyde-functionalized magnetic nanoparticles through amidation reaction. Then, template glycoproteins were immobilized on the boronic acid-modified magnetic nanoparticles via boronate affinity binding. Subsequently, a thin layer of dopamine was formed to coat the surface of magnetic nanoparticles through self-polymerization. After the template glycoproteins were removed, the cavities that can specific bind the template glycoproteins were fabricated. Adopting horseradish peroxidase as model template, the effects of imprinting conditions as well as the properties and performance of the obtained products were investigated. The resultant imprinted materials exhibit highly favorable features, including uniform surface morphology with thin imprinted shell of about 8 nm, super-paramagnetic property, fast kinetics of 40 min, high adsorption capacity of 60.3 mg g(-1), and satisfactory reusability for at least five cycles of adsorption-desorption without obvious deterioration. Meanwhile, the obtained magnetic imprinted nanoparticles could capture target glycoprotein from nonglycoproteins, but also from other glycoproteins because the synergistic selectivity of boronate affinity and imprinting effect. In addition, the facile preparation method shows feasibility in the imprinting of different glycoproteins. (C) 2016 Elsevier B.V. All rights reserved.
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