期刊
TALANTA
卷 147, 期 -, 页码 177-183出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2015.09.055
关键词
Aptasensor; Food-borne pathogen; E. Coli O157:H7; Guanine chemiluminescence; Aptamer; Nanocomposites
资金
- Luminescent MD, LLC [LST-2014-5]
Cost-effective and easy-to-use biosensor was developed for the rapid quantification and monitoring of Escherichia (E.) Coli O157:H7 in sample using E. Coli O157:H7 aptamer, graphene oxide (GO)/iron nano-composites, and guanine chemiluminescence detection. E. Coli O157:H7 aptamer-conjugated 6-carboxyfluorescein (6-FAM) with excellent specificity captured E. Coli O157:H7 in a sample when the mixture was incubated for 1 h at 37 degrees C. Free E. Coli O157:H7 aptamers remaining in sample after the incubation were removed with GO/iron nanocomposites based on the principle of pi-pi stacking interaction between free aptamer and GO/iron nanocomposites. Then, E. Coli O157:H7 bound with aptamer-conjugated 6-FAM in sample emitted strong light when guanine chemiluminescent reagents (e.g., 3,4,5-trimethoxylphenylglyoxal hydrate, Tetra-n-propylammonium hydroxide) were added in the sample. The strength of light emitted in guanine chemiluminescence reaction was proportionally enhanced with the increase of E. Coll O157:H7 concentration. The limit of detection (LOD) of biosensor capable of quantifying E. Coli O157:H7 with good accuracy, precision, and reproducibility was as low as 4.5 x 10(3) cfu/ml. We expect that the rapid analytical system can be applied in the field of food safety as well as public health. (C) 2015 Elsevier B.V. All rights reserved.
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