期刊
TALANTA
卷 147, 期 -, 页码 302-306出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2015.10.004
关键词
DNAzyme; label-free; G-quadruplex; Biosensor; Fluorescence
资金
- National Natural Science Foundation of China [21205068, 21325520, 21221003, J1210040, 21177036]
- Science and Technology Plan of Hunan province [2013WK3015]
DNAzyme-based catalytic beacons have been widely studied for both in vivo and in vitro molecular detection. However, only a few label-free catalytic beacons with excellent analytical performance have been reported so far. In this work, by combining a catalytic DNAzyme for amplified sensing through enzymatic turnover with cleavage-induced G-quadruplex formation, a label-free DNAzyme biosensor was developed for amplified turn-on fluorescence detection of Pb2+ with a detection limit of 3 nM. The method is very competitive compared to many other labeled or label-free methods with or without signal amplification. Due to the inherent specificity of the GR-5 DNAzyme, the method also exhibits excellent selectivity. This biosensor successfully detected Pb2+ in river water samples with high sensitivity and selectivity. Such a method might provide a universal DNAzyme-based sensing platform for sensitive detection of various targets both in environmental and biomedical fields. (C) 2015 Elsevier B.V. All rights reserved.
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