期刊
TALANTA
卷 147, 期 -, 页码 478-484出版社
ELSEVIER
DOI: 10.1016/j.talanta.2015.10.023
关键词
Carbon nanotubes; Restricted access material; Restricted access carbon nanotubes; Lead; Serum; Atomic absorption spectrometry
资金
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG, Belo Horizonte, Brazil) [CDS-PPM-00144-15, CEX-APQ-01556-13]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brasilia, Brazil) [483371/2012-2]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brasilia, Brazil)
Oxidized carbon nanotubes were covered with layers of bovine serum albumin to result in so-called restricted-access carbon nanotubes (RACNTs). This material can extract Pb2+ ions directly from untreated human blood serum while excluding all the serum proteins. The RACNTs have a protein exclusion capacity of almost 100% and a maximum Pb2+ adsorption capacity of 34.5 mg g(-1). High resolution transmission electron microscopy, scanning transmission electron microscopy and energy dispersive spectroscopy were used to confirm the BSA layer and Pb2+ adsorption sites. A mini-column filled with RACNTs was used in an on-line solid phase extraction system coupled to a thermospray flame furnace atomic absorption spectrometry. At optimized experimental conditions, the method has a detection, limit as low as 2.1 mu g L-1, an enrichment factor of 5.5, and inter- and intra-day precisions (expressed as relative standard deviation) of < 8.1%. Recoveries of the Pb2+ spiked samples ranged from 89.4% to 107.3% for the extraction from untreated human blood serum. (C) 2015 Elsevier B.V. All rights reserved.
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