期刊
TALANTA
卷 154, 期 -, 页码 354-359出版社
ELSEVIER
DOI: 10.1016/j.talanta.2016.03.075
关键词
Isothermal Titration Calorimeter; ITC; ITC measurements validation; ITC chemical calibration; Energetics of Ca2+-EDTA chelation
资金
- Spanish Government [CTQ2014-56253-P]
A study about the suitability of the chelation reaction of Ca2+ with ethylenediaminetetraacetic acid (EDTA) as a validation standard for Isothermal Titration Calorimeter measurements has been performed exploring the common experimental variables (buffer, pH, ionic strength and temperature). Results obtained in a variety of experimental conditions have been amended according to the side reactions involved in the main process and to the experimental ionic strength and, finally, validated by contrast with the potentiometric reference values. It is demonstrated that the chelation reaction performed in acetate buffer 0.1 M and 25 degrees C shows accurate and precise results and it is robust enough to be adopted as a standard calibration process. (C) 2016 Elsevier B.V. All rights reserved.
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