期刊
TALANTA
卷 146, 期 -, 页码 457-463出版社
ELSEVIER
DOI: 10.1016/j.talanta.2015.09.006
关键词
SERS; E. coli; Label-free; Discriminant analysis; Single bacterium cell
资金
- China Postdoctoral Science Foundation [2014M550328]
- National Natural Science Foundation of China (NSFC) [21405138, 21505053]
The biosensing for rapid detection of bacteria based on surface-enhanced Raman scattering (SERS) has been widely explored for recent years. It is still a challenge to achieve a high sensitive, reproducible label free detection method for bacteria. In this work, a label-free SERS detection method of Escherichia coli based on incubation with silver colloid was reported. Optimized incubation conditions including shaking speed, time and temperature were used to help construct a rapid SERS method for E. coli analysis. It was found that the enhancement of the Raman signal of E. coli could be achieved to 1.8 x 10(4) cps (counts per second) with high reproducibility. Three strains of E. coli DSM 1116/498/5695 could be successfully discriminated using such SERS method combining discriminant analysis. Finally, the lowest concentration of E coli at 1 x 10(5) cell/mL can be detected by SERS mapping. Thus, our detection method offers higher sensitivity and reproducibility compared to previously reported label free simple-mixing methods, opening an avenue for developing various SERS-based biosensor. (C) 2015 Elsevier B.V. All rights reserved.
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