期刊
SYSTEMATIC AND APPLIED MICROBIOLOGY
卷 39, 期 1, 页码 8-13出版社
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2015.10.006
关键词
Vibrio bivalvicida sp nov.; Orientalis Glade; Bivalve hatchery; Larval pathogen; ANI; DDH
资金
- Ministerio de Economia y Competitividad (Spain) [AGL2011-29765]
- EU [245119]
Three isolates were obtained from cultures of carpet shell clam (Ruditapes decussatus) reared in a bivalve hatchery (Galicia, NW Spain) from different sources: healthy broodstock, moribund larvae and the seawater corresponding to the larval tank. All isolates were studied by a polyphasic approach, including a phylogenetic analysis based on concatenated sequences of the five housekeeping genes ftsZ, gyrB, pyrH, recA and rpoA. The analysis supported their inclusion in the Orientalis Glade of the genus Vibrio, and they formed a tight group separated from the closest relatives: Vibrio tubiashii subsp. europaensis, Vibrio tubiashii subsp. tubiashii and Vibrio orientalis. The percentages of genomic resemblance, including average nucleotide identity, DNA-DNA hybridization and in silico genome-to-genome comparison, between the type strain and the closest relatives were below values for species delineation and confirmed the taxonomic position of the new species, which could be differentiated from the related taxa on the basis of several phenotypic and chemotaxonomic features, including FAME and MALDI-TOF-MS. The pathogenicity of the new species was demonstrated in larvae of R. decussatus, Ruditapes philippinarum, Ostrea edulis and Donax trunculus. The results demonstrated that the strains analyzed represented a novel species in the Orientalis Glade of the genus Vibrio, for which the name Vibrio bivalvicida sp. nov. is proposed, with 605(T) (= CECT 8855(T) = CAIM 1904(T)) designated as the type strain. (C) 2015 Elsevier GmbH. All rights reserved.
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