期刊
SYNTHETIC METALS
卷 212, 期 -, 页码 123-130出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.synthmet.2015.12.012
关键词
Hydrogen peroxide; Hydrogen peroxidase; Biosensor; Poly(aniline-co-N-methylthionine)
资金
- Social Development Foundation of Zhenjiang [SH2011016]
- Natural Science Foundation of Jiangsu Province [BK2012699]
- National Natural Science Foundation of China [51208233]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
In this present work, we developed a novel hydrogen peroxide (H2O2) biosensor fabricated using electrochemical doping to immobilize hydrogen peroxidase (HRP) in a new conducting polymer, poly (aniline-co-N-methylthionine) (PAN-PNMThH). Amperometric detection of H2O2 was evaluated by holding the PAN-PNMThH HRP electrode at -0.25 V (versus saturated calomel electrode (SCE)). PAN-PNMThH showed excellent redox activity and high porosity and acted as an electron transfer mediator. The biosensor had a wide linear response range from 5.0 mu m to 60.0 mM H2O2 with a sensitivity of 35 mA M-1 cm(-2), a detection limit of 3.2 mu M (signal-to-noise ratio of 3) and an apparent Michaelis constant (K-M) of 2.79 mM. The biosensor possessed good analytical performance and storage stability. (C) 2015 Elsevier B.V. All rights reserved.
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