4.7 Article

Key Intermediates in Ribosome Recycling Visualized by Time-Resolved Cryoelectron Microscopy

期刊

STRUCTURE
卷 24, 期 12, 页码 2092-2101

出版社

CELL PRESS
DOI: 10.1016/j.str.2016.09.014

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资金

  1. HHMI
  2. NIH [R01 GM55440, GM29169]
  3. Swedish Research Council [2015-04682]
  4. Knut and Alice Wallenberg Foundation
  5. RiboCORE [KAW 2009.0251]
  6. Liljewalchs travel stipend (Uppsala University)
  7. Swedish Research Council [2015-04682] Funding Source: Swedish Research Council

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Upon encountering a stop codon on mRNA, polypeptide synthesis on the ribosome is terminated by release factors, and the ribosome complex, still bound with mRNA and P-site-bound tRNA (post-termination complex, PostTC), is split into ribosomal subunits, ready for a new round of translational initiation. Separation of post-termination ribosomes into subunits, or ribosome recycling,'' is promoted by the joint action of ribosome-recycling factor (RRF) and elongation factor G (EF-G) in a guanosine triphosphate (GTP) hydrolysis-dependent manner. Here we used a mixing-spraying-based method of time-resolved cryo-electron microscopy (cryo-EM) to visualize the short-lived intermediates of the recycling process. The two complexes that contain (1) both RRF and EF-G bound to the PostTC or (2) deacylated tRNA bound to the 30S subunit are of particular interest. Our observations of the native form of these complexes demonstrate the strong potential of time-resolved cryo-EM for visualizing previously unobservable transient structures.

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