期刊
STEM CELLS
卷 34, 期 8, 页码 2008-2015出版社
WILEY
DOI: 10.1002/stem.2403
关键词
Human; Pluripotent stem cells; Heart; Drug testing; Cardiac disease modelling; Cardiomyocyte maturation; Cardiomyocyte physiology
资金
- European Research Council [ERCAdG 323182 STEMCARDIOVASC]
- GSK-NC3R CrackIt programme INPULSE
- National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) [NC/C013202/1, NC/C013105/1] Funding Source: researchfish
Cardiomyocytes from human pluripotent stem cells (hPSC) are of growing interest as models to understand mechanisms underlying genetic disease, identify potential drug targets and for safety pharmacology as they may predict human relevant effects more accurately and inexpensively than animals or other cell models. Crucial to their optimal use are accurate methods to quantify cardiomyocyte phenotypes accurately and reproducibly. Here, we review current methods for determining biophysical parameters of hPSC-derived cardiomyocytes (hPSC-CMs) that recapitulate disease and drug responses. Even though hPSC-CMs as currently available are immature, various biophysical methods are nevertheless already providing useful insights into the biology of the human heart and its maladies. Advantages and limitations of assays currently available looking toward applications of hPSC-CMs are described with examples of how they have been used to date. This will help guide the choice of biophysical method to characterize healthy cardiomyocytes and their pathologies in vitro.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据