期刊
STEM CELL RESEARCH
卷 17, 期 1, 页码 6-15出版社
ELSEVIER
DOI: 10.1016/j.scr.2016.04.018
关键词
Acute myocardial infarction; Adipose tissue-derived stem cells; Targeting; Microbubbles; StemBells; Acoustic radiation force
资金
- Dutch Technology Foundation STW [10507]
Successful stem cell therapy after acute myocardial infarction (AMI) is hindered by lack of engraftment of sufficient stemcells at the site of injury. Wedesigned a novel technique to overcome this problemby assembling stem cell-microbubble complexes, named 'StemBells'. StemBells were assembled through binding of dual-targetedmicrobubbles (similar to 3 mu m) to adipose-derived stemcells (ASCs) via a CD90 antibody. StemBells were targeted to the infarct area via an ICAM-1 antibody on the microbubbles. StemBells were characterized microscopically and by flow cytometry. The effect of ultrasound on directing StemBells towards the vessel wall was demonstrated in an in vitro flow model. In a rat AMI-reperfusion model, StemBells or ASCs were injected one week post-infarction. A pilot study demonstrated feasibility of intravenous StemBell injection, resulting in localization in ICAM-1-positive infarct area three hours post-injection. In a functional study five weeks after injection of StemBells cardiac function was significantly improved compared with controls, as monitored by 2D-echocardiography. This functional improvement neither coincided with a reduction in infarct size as determined by histochemical analysis, nor with a change in anti-and pro-inflammatory macrophages. In conclusion, the StemBell technique is a novel and feasible method, able to improve cardiac function post-AMI in rats. (C) 2016 The Authors. Published by Elsevier B.V.
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