期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 152, 期 -, 页码 272-277出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2015.07.003
关键词
L-Tryptophan (L-Trp); Adenine; Fluorescence recovery; DNA
类别
资金
- National Natural Science Foundation of China [21175109]
- Special Fund of Chongqing Key Laboratory (Chongqing Science and Technology Commission, Chongqing People's Municipal Government)
- Fundamental Research Funds for the Central Universities [XDJK2013A022]
A simple and sensitive method for determination of adenine was developed based on fluorescence quenching and recovery of L-Tryptophan (L-Trp). The fluorescence of L-Trp could efficiently quenched by copper ion compared with other common metal ions. Upon addition of adenine (Ade) in L-Trp-Cu(II) system, the fluorescence was reoccurred. Under the optimum conditions, the recovery fluorescence intensity was linearly correlated with the concentration of adenine in the range from 0.34 to 25.01 mu mol L-1, with a correlation coefficient (R-2) of 0.9994. The detection limit (3 sigma/k) was 0.046 mu mol L-1, indicating that this method could applied to detect trace adenine. In this study, amino acids including L-Trp, D-Trp, L-Tyr, D-Tyr, L-Phe, D-Phe were investigated and only L-Trp could well chelated copper ion. Additionally, the mechanism of quench and recovery also were discussed and the method was successfully applied to detect the adenine in DNA with satisfactory results. (C) 2015 Elsevier B.V. All rights reserved.
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