期刊
SOIL BIOLOGY & BIOCHEMISTRY
卷 96, 期 -, 页码 16-19出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.soilbio.2016.01.008
关键词
DNA metabarcoding; DNA extraction protocol; Tropical forest; Multi-taxa biodiversity
类别
资金
- METABAR project [ANR-11-BSV7-0020]
- Investissement d'Avenir grants - Agence Nationale de la Recherche [ANR-10-LABX-25-01, ANR-10-LABX-0041, ANR-11-INBS-0001]
DNA metabarcoding on soil samples is increasingly used for large-scale and multi-taxa biodiversity studies. However, DNA extraction may be a major bottleneck for such wide uses. It should be cost/time effective and allow dealing with large sample volumes so as to maximise the representativeness of both micro- and macro-organisms diversity. Here, we compared the performances of a fast and cheap extracellular DNA extraction protocol with a total DNA extraction method in retrieving bacterial, eukaryotic and plant diversity from tropical soil samples of ca. 10 g. The total DNA extraction protocol yielded more high-quality DNA. Yet, the extracellular DNA protocol provided similar diversity assessments although it presented some differences in clades relative abundance and undersampling biases. We argue that extracellular DNA is a good compromise between cost, labor, and accuracy for high throughput DNA metabarcoding studies of soil biodiversity. (C) 2016 Elsevier Ltd. All rights reserved.
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