期刊
SMALL
卷 12, 期 24, 页码 3292-3301出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201600393
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资金
- BOF (Ghent University)
- FWO
- FP-7 EU project DINaMIT
- Hercules grant [AKUL2011/30]
- Laser Lab Europe (EC's Seventh Framework Programme) [284464]
- Photonics4Life Network of Excellence
- European Regional Development Fund
- MINECO Severo Ochoa grant [SEV-2015-0522]
- Fundacio Cellex Barcelona
- Research Foundation - Flanders (FWO)
Exosome-like vesicles (ELVs) are a novel class of biomarkers that are receiving a lot of attention for the detection of cancer at an early stage. In this study the feasibility of using a surface enhanced Raman spectroscopy (SERS) based method to distinguish between ELVs derived from different cellular origins is evaluated. A gold nanoparticle based shell is deposited on the surface of ELVs derived from cancerous and healthy cells, which enhances the Raman signal while maintaining a colloidal suspension of individual vesicles. This nanocoating allows the recording of SERS spectra from single vesicles. By using partial least squares discriminant analysis on the obtained spectra, vesicles from different origin can be distinguished, even when present in the same mixture. This proof-of-concept study paves the way for noninvasive (cancer) diagnostic tools based on exosomal SERS fingerprinting in combination with multivariate statistical analysis.
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