Applications of choline amino acid ionic liquid in extraction and separation of flavonoids and pectin from ponkan peels

Choline leucine ionic liquid solution was chosen to simultaneously extract flavonoids and pectin from ponkan peels, which were subsequently isolated by the corresponding K3PO4-based aqueous biphasic systems (ABS). Under optimized conditions of 54% solution for 100 min extraction at liquid-solid ratio 17, the predicted yields of flavonoids and pectin achieved 1.33% and 13.42%, respectively, the flavonoids determined directly after extraction and the pectin after ABS purification, with the two corresponding measured yields of 1.26% +/- 0.04% and 12.85% +/- 0.51%. Under optimal extraction, crude flavonoids contained maximal total flavonoid content of about 20% +/- 1% after purification of the ABS involving 25% K3PO4 and dilution precipitation. What was directly isolated from K3PO4-rich phase was presumed to be a modified pectin.