期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 232, 期 -, 页码 84-90出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.02.106
关键词
Graphene; Biosensor; Hybrid nanomaterial; Magnetic nanoparticles; Fish freshness
资金
- Spanish Ministry of Economy and Competitiveness [CTQ2014-58989-P, CTQ2011-24355, CTQ2015-71936-REDT, CTQ2012-34238]
- Comunidad de Madrid, Programme NANOAVANSENS [S2013/MIT-3029]
The assembly of a novel layer-by-layer biosensor architecture using hybrid nanomaterials is explored for the construction of an amperometric enzyme biosensors. The nanostructured sensing interface was prepared with poly(dopamine)-modified magnetic nanoparticles which were covalently coated with four-generation ethylenediamine core polyamidoamine G-4 dendrimers and further decorated with platinum nanoparticles. This nanohybrid was fully characterized and further layered on glassy carbon electrodes coated with a graphene oxide-carboxymethylcellulose hybrid nanomaterial through electrostatic interactions. The nanostructured surface was then employed as scaffold for the covalent immobilization of the enzyme xanthine oxidase through a glutaraldehyde-mediated cross-linking. The enzyme electrode allowed the amperometric detection of xanthine in the 50 nM-12 mu M range, with a high sensitivity of 140 mA/M cm(2) and low detection limit of 13 nM. The biosensor exhibited high reproducibility and repeatability, and was successfully tested for the quantification of xanthine in fish samples. (C) 2016 Elsevier B.V. All rights reserved.
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