期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 232, 期 -, 页码 619-624出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.04.014
关键词
Fluorescent probe; Maleimide group; Selective; Thiol; Bioimaging
资金
- National Natural Science Foundation of China [21472118]
- Program for the Top Young and Middle-aged Innovative Talents of Higher Learning Institutions of Shanxi (TYMIT) [2013802]
- talents Support Program of Shanxi Province [2014401]
Up to date, the design of highly selective and fast response probes for Cys, Hcy, and GSH is still a challenge and very few examples have been reported so far. In this study, we synthesized a 1,8-naphthalimide-based probe bearing maleimide group to selectively detect thiol. After introduce thiol, the fluorescence intensity of probe showed significant enhancement, but other amino acids did not generate any change. The kinetic study showed that the reaction was complete within 120 s for Cys, Hcy and GSH, indicating that probe reacts rapidly with thiol under the experimental conditions. Furthermore, the ability of probe to detect thiol in living cells via an enhancement of the fluorescence was proved. (C) 2016 Elsevier B.V. All rights reserved.
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