A ratiometric fluorescent probe for fast detection of hydrogen sulfide and recognition of biological thiols

A new fluorescent probe based on the cleavage of disulfide bond and subsequent intramolecular cyclization process was designed for the detection of H2S. The selectivity was guaranteed by use of the unique nucleophilicity of H2S. The impacts of biological thiols were neglectable and biological thiols could lead to a fluorescence emission band different from H2S with longer response time. The probe was also applied to detect H2S in living cells successfully. (C) 2016 Elsevier B.V. All rights reserved.