期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 230, 期 -, 页码 556-558出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.02.107
关键词
ATP detection; AIE; DNA aptasensor; Water-soluble carbon nanotubes
资金
- National Basic Research Program of China [2013CB834702]
- Natural Science Foundation of China [51373063, 21204027, 21221063]
- Research Fund for the Doctoral Program of Higher Education of China [20120061120016]
- Graduate Innovation Fund of Jilin University [2014082]
A label-free and turn-on fluorescent ATP detection is reported with a ss-DNA aptamer as the recognition part, an AIE molecule as the fluorescent probe, and water-soluble carbon nanotubes as the selective part with the fluorescent quenching effect. The specific binding between the aptamer and target ATP makes the aptamer released from the water-soluble carbon nanotubes. As a consequence, the AIE-active probes, attaching on the surface of ss-DNA, recover to give fluorescence and present a strong emission. The introduction of water-soluble carbon nanotubes allows creating low background fluorescence, which is a great advantage for ATP detection. (C) 2016 Elsevier B.V. All rights reserved.
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