期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 222, 期 -, 页码 264-269出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.08.057
关键词
Electrochemiluminescence sensor; Ru-silica nanoparticles; Molecularly imprinted polymers; Ochratoxin A
资金
- National Natural Science Foundation of China [21075029, 31501568, 21375033]
- Natural Science Fund for Creative Research Groups of Hubei Province of China [2011CDA111, 2014CFA015]
- Natural Science Foundation of Hubei Province [2015CFA124]
- Program for Excellent Youth Scholars of Innovative Research Team by Hubei Provincial Department of Education [T201101]
An electrochemiluminescence (ECL) sensor based on Ru(bpy)(3)(2+)-doped silica nanoparticles (RuSi NPs) combined with molecularly imprinted polymer (MIP) has been developed for the determination of ochratoxin A (OTA). The sensor was fabricated by remodification of RuSi NPs with a thin film of molecularly imprinted polymer, which provided the specific binding sites for OTA. The process of template elution and rebinding acted as a gate to control the flux of probes, which passed through the cavities and reacted with Ru(bpy)(3)(2+) immobilized on the electrode surface to emit ECL signal. The ECL intensity decreased with the OTA molecules rebound in MIP. The as-prepared sensor exhibited a very high sensitivity and excellent selectivity to the target molecule. The Delta I-ECL depended linearly on the logarithm of the OTA concentration in the range from 0.1 pg/mL to 14.76 ng/mL with lower detection limit of 0.027 pg/mL (S/N = 3). The MIP-ECL sensor enabled the successful application of this method to determine OTA in corn. (C) 2015 Elsevier B.V. All rights reserved.
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