期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 228, 期 -, 页码 180-184出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.01.028
关键词
Ruthenium (II)-graphene oxide; NAD(+)/NADH; 3-hydroxybutyrate dehydrogenase; Biosensor platform; BHBA detection
资金
- Natural Sciences and Engineering Research Council of Canada [400929]
- Ontario Ministry of Research and Innovation [051455]
- Dairy Farmers of Ontario [051520]
Regular monitoring of circulating serum p-hydroxybutyrate is vital for dairy cattle health management. Herein, an electrochemical biosensor platform for rapid detection of p-hydroxybutyrate is described that is simple to operate. The biosensor platform is constructed by integrating the covalently functionalized ruthenium (II)-graphene oxide/NAD' composite with subsequent modification of enzyme, 3-hydroxybutyrate dehydrogenase. As-functionalized biosensor platform display enhanced redox behavior at the interface with a current sensitivity of 22 +/- 2.51 mu A mM(-1). Immobilization of enzyme 3-hydroxybutyrate dehydrogenase makes the sensor platform selective to p-hydroxybutyrate, which induces the concentration dependent amperometric response suitable for field -deployable application. Further, practical applicability of the proposed biosensor platform is tested with serum samples of various p-hydroxybutyrate concentrations. In addition to better selectivity, the demonstrated enzyme integrated hybrid nanocomposite biosensor exhibit rapid response time (less than 1 min), providing a promising approach for on -farm diagnostics. (C) 2016 Elsevier B.V. All rights reserved.
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