期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 222, 期 -, 页码 1066-1072出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.09.049
关键词
Fluorescence aptasensor; Chloramphenicol; Exonuclease-assisted target recycling; FRET; SiO2@Au nanotracer
资金
- National Natural Science Foundation of China [31070866]
- Natural Science Foundation of Zhejiang [LY13C200017, Y15B050008]
- Natural Science Foundation of Ningbo [2013A610241, 2013A610163, 2014A610184]
- Science and Technology Project of Zhejiang [2013C37033]
- State Administration of Grain [201313010]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- K.C. Wong Magna Fund in Ningbo University
In this work, a fluorescence aptasensor has been developed to detect trace chloramphenicol based on FRET and exonuclease-assisted target recycling. Firstly, the composite probe for CAP was prepared through the immunoreactions between the capture probe based on dsDNA antibody labeled on Fe3O4@Au nanoparticles and the nanotracer using double strand aptamer (aptamer hybrid with its complementary DNA) labeled on core-shell SiO2@Au. When the composite probe solution was mixed with CAP, the aptamer on the nanotracer preferentially bounded with CAP, and then released cDNA-SiO2@Au and CAP-Apt complex to the supernatant. This is because anti-DNA on capture probes can't recognize the ssDNA. The exonuclease I (Exo I) added into the supernatant can further digest the aptamer-CAP from the 3'-end of aptamer and release CAP again, which can further participate in new cycle to react with the probes. The supernatant containing numerous SiO2@Au could efficiently quench the fluorescence response of CdSe QDs by FRET. Experimental results showed the CAP detection owning a linearity range of 0.001-10 ng mL(-1) and detection of limit (LOD) of 0.0002 ng mL(-1). Besides, the results of our method for CAP detection in the fish samples agreed well with those from ELISA, verifying its accuracy and reliability. (C) 2015 Elsevier B.V. All rights reserved.
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