期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 233, 期 -, 页码 394-401出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.04.096
关键词
Enrofloxacin; Upconversion nanoparticles; Aptamer; Sensor; Magnetic nanoparticles
资金
- Cultivation Plan for Youth Agricultural Science and Technology Innovative Talents of Liaoning Province [2015001]
- National Natural Science Foundation of China [31471639]
- National Key Technologies R & D Program of China during the 12th Five-Year Plan Period [2012BAD29B06]
In this study, aptamer-functionalized magnetic Fe3O4 conjugated with upconversion nanoparticles (UCNPs) were used as hybridization nanoprobes to quantify trace enrofloxacin (ENR) in food samples. Owing to the autofluorescence-free nature of UCNPs, an ENR-responsive aptamer, and the use of magnetic separation to further reduce background signals, our sensing system enabled rapid and highly sensitive detection of trace ENR in real samples. The proposed sensing method was validated in terms of selectivity, linearity, accuracy, precision, and sensitivity; and then was applied to the analysis of four different fish samples. Recoveries between 85.1% and 98.5%, and relative standard deviation (RSD) values in the range of 2.4% to 5.0% and from 3.0% to 4.3%, for intra- and inter-day tests, respectively, were obtained, with the limits of detection and quantification of 0.06 and 0.20 ng/mL, respectively. Therefore, the good validation and application results that we obtained indicate that the sensing method is suitable for the quantification of target ENRs in real samples, and show great potential for wide-ranging application in bioassays by simply changing the type of aptamer. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据