期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 230, 期 -, 页码 779-784出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.02.143
关键词
Polymerase chain reaction; Natural convective; Capillary electrophoresis
资金
- National Natural Science Foundation of China [21205078]
- Research Fund for the Doctoral Program of Higher Education of China [20123120110002]
- JSPS, Japan [25600049]
- Grants-in-Aid for Scientific Research [15H03827, 25600049] Funding Source: KAKEN
Rapid, low cost, and portable polymerase chain reaction system (PCR) can advance the diagnosis of infectious disease, especially for the third-world countries. Herein, we developed a compact PCR system based on Rayleigh-Benard convection (RB-PCR). The optimal geometry parameters of the RB-PCR cell was obtained by finite element analysis. The amplification efficiency was improved by adding polyvinyl pyrrolidone into the PCR reagent and evaluated by detection of PCR products in capillary electrophoresis. Results demonstrate that lambda-DNA was successfully amplified within 15 min, and the PCR products was identified within 6.0 min. 0.1% PVP (10 mg/9 ml) in PCR solution offers the best amplification efficiency for RB-PCR. The device is easy to assemble, and it is well suited for point of care applications. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据