Rhamnolipid-enhanced aerobic biodegradation of triclosan (TCS) by indigenous microorganisms in water-sediment systems

Bioremediation of triclosan (TCS) is a challenge because of its low bioavailability persistence in the environment and recalcitrance to remediation efforts. Rhamnolipid (RL) was used to enhance TCS biodegradation by indigenous microbes in an aerobic water-sediment system However, knowledge of the effects of TCS on the bacterial community and environmental factors in an RL-enhanced, TCS-degrading system are lacking. Therefore, in this study, the influence of environmental factors on RL-enhanced biodegradation of TCS was investigated by single factor experiments, and shifts in aerobic TCS-degrading bacterial populations, with and without RL, were analyzed by high-throughput sequencing technology. The results showed that aerobic biodegradation of TCS was significantly promoted by the addition of RL. Environmental conditions, which included RL addition (0.125-0.5 g/L), medium concentrations of TCS (<90 mu g/g), water disturbance, elevated temperature, ionic strength (0.001-0.1 mol/L NaCI) and weak alkaline environments (pH 8-9), were monitored. High concentrations of TCS had a remarkable influence on the bacterial community structure, and this influence on the distribution proportion of the main microorganisms was strengthened by RL addition. Alpha-proteobacteria (e.g., Sphingomonadaceae and Caulobacteraceae) might be resistant to TCS or even capable of TCS biodegradation, while Sphingobacteria, Beta-and Delta-proteobacteria were sensitive to TCS toxicity. This research provides ecological information on the degradation efficiency and bacterial community stability in RL-enhanced bioremediation of TCS-polluted aquatic environments. (C) 2016 Elsevier B.V. All rights reserved.