期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 550, 期 -, 页码 1163-1170出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2015.11.084
关键词
Lead; Cadmium; Placental tissue; Differential proteomics; Fetal development
资金
- Natural Science Foundation of China, NSFC [21577084, 21377077]
- Science and Technology Planning Project of Guangdong Province of China [20138051000078]
- Natural Science Foundation of Guangdong Province of China [2015A030313435]
Prenatal exposure to lead (Pb) and cadmium (Cd) has been associated with a series of physiological problems resulting in fetal growth restriction. We aimed to investigate the effects of Pb and Cd exposure on placental function and the potential mechanisms involved in fetal development. Placental specimens and questionnaires were collected from an c-waste area and a reference area in China. Two-dimensional electrophoresis combined with MALDI-TOF-MS/MS and molecular network relationship were performed to analyze differentially expressed proteins using a compositing sample pool Compared with the reference group, the exposed group exhibited significantly higher levels of placental Pb and Cd (p < 0.01), shorter body length and higher gestational age (p < 0.01). After bivariate adjustment in a linear regression model, decreases of 205.05 gin weight and 0.44 cm in body length were associated with a 10 ng/g wt increase in placental Cd. Pb showed a negative trend but lacked statistical significance. Proteomic analysis showed 32 differentially-expressed proteins and were predominantly involved in protein translocation, cytoskeletal structure, and energy metabolism. Fumarate hydratase was down regulated in the exposed placenta tissues and validated by ELISA. Alterations in placental proteome suggest that imbalances in placental mitochondria respiration might be a vital pathway targeting fetal growth restriction induced by exposure to Cd. (C) 2015 Elsevier B.V. All rights reserved.
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