期刊
SCIENCE CHINA-LIFE SCIENCES
卷 60, 期 3, 页码 264-270出版社
SCIENCE PRESS
DOI: 10.1007/s11427-016-0183-1
关键词
replication; histone; TXR1; Tetrahymena; substrate preference
类别
资金
- Natural Science Foundation of China [31470064, 31522051]
- National Institutes of Health [R01GM087343]
- Qingdao National Laboratory for Marine Science and Technology, China [2015ASTP]
- Qingdao government [15-12-1-1-jch]
- Direct For Biological Sciences [1411565] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience [1411565] Funding Source: National Science Foundation
DNA replication elongation is tightly controlled by histone-modifying enzymes. Our previous studies showed that the histone methytransferase TXR1 (Tetrahymena Trithorax related protein 1) specifically catalyzes H3K27 monomethylation and affects DNA replication elongation in Tetrahymena thermophila. In this study, we investigated whether TXR1 has a substrate preference to the canonical H3 over the replacement variant H3.3. We demonstrated by histone mutagenesis that K27Q mutation in H3.3 further aggravated the replication stress phenotype of K27Q mutation in canonical H3, supporting H3.3 as a physiologically relevant substrate of TXR1. This result is in apparent contrast to the strong preference for canonical H3 recently reported in Arabidopsis homologues ATXR5 and ATXR6, and further corroborates the role of TXR1 in DNA replication.
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