期刊
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
卷 17, 期 1, 页码 644-649出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14686996.2016.1223516
关键词
DNA methylation; single DNA molecule; nanochannel; DNA contraction
资金
- Cabinet Office, Government of Japan
- Japan Society for the Promotion of Science (JSPS) through the Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program)
- ImPACT Program of the Council for Science, Technology and Innovation (Cabinet Office, Government of Japan)
- Nanotechnology Platform Program (Molecule and Material Synthesis) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
- JSPS [16H02091]
- MEXT [26107709]
- PRESTO, Japan Science and Technology Agency (JST)
- Grants-in-Aid for Scientific Research [16H01692, 26107709, 16H02091, 15K13288] Funding Source: KAKEN
DNA methylation is a stable epigenetic modification, which is well known to be involved in gene expression regulation. In general, however, analyzing DNA methylation requires rather time consuming processes (24-96 h) via DNA replication and protein modification. Here we demonstrate a methodology to analyze DNA methylation at a single DNA molecule level without any protein modifications by measuring the contracted length and relaxation time of DNA within a nanochannel. Our methodology is based on the fact that methylation makes DNA molecules stiffer, resulting in a longer contracted length and a longer relaxation time (a slower contraction rate). The present methodology offers a promising way to identify DNA methylation without any protein modification at a single DNA molecule level within 2 h. [GRAPHICS] .
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