期刊
CIRCULATION
卷 132, 期 12, 页码 1081-1093出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/CIRCULATIONAHA.114.014821
关键词
autophagy; fibroblasts; receptors; transforming growth factor beta; sirtuins; wound healing
资金
- Okukubo Memorial Fund for Medical Research at Kumamoto University School of Medicine
- Tokyo Biochemical Research Foundation
- Japan Research Promotion Society for Cardiovascular Diseases
- Public Trust Cardiovascular Research Fund
- Ministry of Education, Science, and Culture, Japan [B-24790768]
- Excellence Initiative Cardiopulmonary System
- University of Giessen-Marburg Lung Center (UGMLC)
- Cell and Gene Therapy Center (CGT)
- German Center for Cardiovascular Research (DZHK)
- [B-20706717]
- Grants-in-Aid for Scientific Research [25461361, 26461364, 25293186, 25293212, 24790768, 26860575] Funding Source: KAKEN
Background Sirt7, 1 of the 7 members of the mammalian sirtuin family, promotes oncogenic transformation. Tumor growth and metastasis require fibrotic and angiogenic responses. Here, we investigated the role of Sirt7 in cardiovascular tissue repair process. Methods and Results In wild-type mice, Sirt7 expression increased in response to acute cardiovascular injury, including myocardial infarction and hind-limb ischemia, particularly at the active wound healing site. Compared with wild-type mice, homozygous Sirt7-deficient (Sirt7(-/-)) mice showed susceptibility to cardiac rupture after myocardial infarction, delayed blood flow recovery after hind-limb ischemia, and impaired wound healing after skin injury. Histological analysis showed reduced fibrosis, fibroblast differentiation, and inflammatory cell infiltration in the border zone of infarction in Sirt7(-/-) mice. In vitro, Sirt7(-/-) mouse-derived or Sirt7 siRNA-treated cardiac fibroblasts showed reduced transforming growth factor- signal activation and low expression levels of fibrosis-related genes compared with wild-type mice-derived or control siRNA-treated cells. These changes were accompanied by reduction in transforming growth factor receptor I protein. Loss of Sirt7 activated autophagy in cardiac fibroblasts. Transforming growth factor- receptor I downregulation induced by loss of Sirt7 was blocked by autophagy inhibitor, and interaction of Sirt7 with protein interacting with protein kinase-C was involved in this process. Conclusion Sirt7 maintains transforming growth factor receptor I by modulating autophagy and is involved in the tissue repair process.
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