期刊
SCIENCE
卷 352, 期 6289, 页码 1098-1101出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaf3165
关键词
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资金
- European Commission [FP7-KBBE-2013-7-613549]
- Austrian Science Fund (project BioToP) [FWF W1224]
- BMWFW (Austrian Federal Ministry of Science, Research and Economy) IGS BioNanoTech
- Austrian Academy of Sciences
Ninety percent of lignocellulose-degrading fungi contain genes encoding lytic polysaccharide monooxygenases (LPMOs). These enzymes catalyze the initial oxidative cleavage of recalcitrant polysaccharides after activation by an electron donor. Understanding the source of electrons is fundamental to fungal physiology and will also help with the exploitation of LPMOs for biomass processing. Using genome data and biochemical methods, we characterized and compared different extracellular electron sources for LPMOs: cellobiose dehydrogenase, phenols procured from plant biomass or produced by fungi, and glucose-methanol-choline oxidoreductases that regenerate LPMO-reducing diphenols. Our data demonstrate that all three of these electron transfer systems are functional and that their relative importance during cellulose degradation depends on fungal lifestyle. The availability of extracellular electron donors is required to activate fungal oxidative attack on polysaccharides.
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