期刊
SCIENCE
卷 351, 期 6274, 页码 684-687出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aad3436
关键词
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资金
- National Institute of General Medical Sciences [R01 GM046451]
- NSF [IOS-1339348]
- Indiana University Office of the Vice Provost for Research Faculty Research Support Program
Maintaining high crop yields in an environmentally sustainable manner requires the development of disease-resistant crop varieties. We describe a method to engineer disease resistance in plants by means of an endogenous disease resistance gene from Arabidopsis thaliana named RPS5, which encodes a nucleotide-binding leucine-rich repeat (NLR) protein. RPS5 is normally activated when a second host protein, PBS1, is cleaved by the pathogen-secreted protease AvrPphB. We show that the AvrPphB cleavage site within PBS1 can be substituted with cleavage sites for other pathogen proteases, which then enables RPS5 to be activated by these proteases, thereby conferring resistance to new pathogens. This decoy approach may be applicable to other NLR proteins and should enable engineering of resistance in plants to diseases for which we currently lack robust genetic resistance.
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