期刊
SCIENCE
卷 355, 期 6321, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aal1880
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资金
- European Molecular Biology Organization [ALTF 51-2014]
- Swiss National Science Foundation [155515]
- Natural Sciences and Engineering Research Council of Canada
- Robertson Foundation
- Alfred P. Sloan Foundation
- Irma T. Hirschl Trust
- Alexandrine and Alexander L. Sinsheimer Fund
- Human Frontier Science Program
The small subunit (SSU) processome, a large ribonucleoprotein particle, organizes the assembly of the eukaryotic small ribosomal subunit by coordinating the folding, cleavage, and modification of nascent pre-ribosomal RNA (rRNA). Here, we present the cryo-electron microscopy structure of the yeast SSU processome at 5.1-angstrom resolution. The structure reveals how large ribosome biogenesis complexes assist the 5' external transcribed spacer and U3 small nucleolar RNA in providing an intertwined RNA-protein assembly platform for the separate maturation of 18S rRNA domains. The strategic placement of a molecular motor at the center of the particle further suggests a mechanism for mediating conformational changes within this giant particle. This study provides a structural framework for a mechanistic understanding of eukaryotic ribosome assembly in the model organism Saccharomyces cerevisiae.
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