期刊
SCIENCE
卷 354, 期 6314, 页码 890-893出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aag2347
关键词
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资金
- VILLUM Research Center for Plant Plasticity
- bioSYNergy program of Center for Synthetic Biology (University of Copenhagen Excellence Program for Interdisciplinary Research)
- European Research Council [ERC-2012-ADG_20120314]
- VILLUM Foundation
- VILLUM Foundation [95-300-73023]
- P4FIFTY Marie Curie Initial Training Network (European Union)
- Innovation Fund Denmark [001-2011-4]
- National Research Foundation of Singapore [NRFI2015-05]
- Biomedical Research Council-Science and Engineering Research Council from the Singapore Agency for Science, Technology and Research [112 148 0006]
- Biological and Biotechnology Science Research Council [BB/J017310/1, BB/K004441/1]
- Villum Fonden [00010099, 00007523] Funding Source: researchfish
Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling. We used in planta fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to study functional and structural characteristics of the metabolon. Understanding the regulation of biosynthetic metabolons offers opportunities to optimize synthetic biology approaches for efficient production of high-value products in heterologous hosts.
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