期刊
SCIENCE
卷 352, 期 6289, 页码 1113-1116出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaf5124
关键词
-
资金
- Howard Hughes Medical Institute
- NIH [R01 GM102308, F32 GM116318]
Linkage and association studies have mapped thousands of genomic regions that contribute to phenotypic variation, but narrowing these regions to the underlying causal genes and variants has proven much more challenging. Resolution of genetic mapping is limited by the recombination rate. We developed a method that uses CRISPR (clustered, regularly interspaced, short palindromic repeats) to build mapping panels with targeted recombination events. We tested the method by generating a panel with recombination events spaced along a yeast chromosome arm, mapping trait variation, and then targeting a high density of recombination events to the region of interest. Using this approach, we fine-mapped manganese sensitivity to a single polymorphism in the transporter Pmr1. Targeting recombination events to regions of interest allows us to rapidly and systematically identify causal variants underlying trait differences.
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