期刊
RNA BIOLOGY
卷 14, 期 8, 页码 1018-1027出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2016.1239009
关键词
Chemical ligation; circularization; circRNA; enzymatic ligation; in vitro transcription; kinase; ligase; RNA synthesis; transcription priming
Over the past 2 decades, different types of circular RNAs have been discovered in all kingdoms of life, and apparently, those circular species are more abundant than previously thought. Apart from circRNAs in viroids and viruses, circular transcripts have been discovered in rodents more than 20 y ago and recently have been reported to be abundant in many organisms including humans. Their exact function remains still unknown, although one may expect extensive functional studies to follow the currently dominant research into identification and discovery of circRNA by sophisticated sequencing techniques and bioinformatics. Functional studies require models and as such methods for preparation of circRNA in vitro. Here, we will review current protocols for RNA circularization and discuss future prospects in the field.
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