期刊
RNA BIOLOGY
卷 13, 期 9, 页码 895-915出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2016.1208332
关键词
Antisense; Listeria monocytogenes; multiplicity; post-transcriptional regulation; sRNAs
资金
- Danish Council for Independent Research \ Natural Sciences [12-124735]
- VILLUM FONDEN
- Lundbeck Foundation
- Novo Nordisk Foundation
- Center National de la Recherche Scientifique (CNRS)
- Lundbeck Foundation [R100-2011-9234] Funding Source: researchfish
- Novo Nordisk Fonden [NNF14OC0011429] Funding Source: researchfish
Multicopy small RNAs (sRNAs) have gained recognition as an important feature of bacterial gene regulation. In the human pathogen Listeria monocytogenes, 5 homologous sRNAs, called LhrC1-5, control gene expression by base pairing to target mRNAs though 3 conserved UCCC motifs common to all 5 LhrCs. We show here that the sRNAs Rli22 and Rli33-1 are structurally and functionally related to LhrC1-5, expanding the LhrC family to 7 members, which makes it the largest multicopy sRNA family reported so far. Rli22 and Rli33-1 both contain 2 UCCC motifs important for post-transcriptional repression of 3 LhrC target genes. One such target, oppA, encodes a virulence-associated oligo-peptide binding protein. Like LhrC1-5, Rli22 and Rli33-1 employ their UCCC motifs to recognize the Shine-Dalgarno region of oppA mRNA and prevent formation of the ribosomal complex, demonstrating that the 7 sRNAs act in a functionally redundant manner. However, differential expression profiles of the sRNAs under infection-relevant conditions suggest that they might also possess non-overlapping functions. Collectively, this makes the LhrC family a unique case for studying the purpose of sRNA multiplicity in the context of bacterial virulence.
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