期刊
RNA BIOLOGY
卷 14, 期 1, 页码 90-103出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2016.1256533
关键词
Fluorescence spectroscopy; genomic RNA selection; high affinity binding site; HIV-1; Pr55(Gag); protein-RNA interaction; stoichiometry
资金
- Agence Nationale de la Recherche sur le SIDA et les Hepatites Virales (ANRS)
- SIDACTION
- US National Institutes of Health and Australian NHMRC
- ARC
- Egyptian Ministry of Higher Education and Scientific Research
- Initiative d'excellence (IDEX: Par dela les frontieres, l'Universite de Strasbourg)
The HIV-1 Pr55(Gag) precursor specifically selects genomic RNA (gRNA) from a large variety of cellular and spliced viral RNAs (svRNAs), however the molecular mechanisms of this selective recognition remains poorly understood. To gain better understanding of this process, we analyzed the interactions between Pr55(Gag) and a large panel of viral RNA (vRNA) fragments encompassing the main packaging signal (Psi) and its flanking regions by fluorescence spectroscopy. We showed that the gRNA harbors a high affinity binding site which is absent from svRNA species, suggesting that this site might be crucial for selecting the HIV-1 genome. Our stoichiometry analysis of protein/RNA complexes revealed that few copies of Pr55(Gag) specifically associate with the 5 region of the gRNA. Besides, we found that gRNA dimerization significantly impacts Pr55(Gag) binding, and we confirmed that the internal loop of stem-loop 1 (SL1) in Psi is crucial for specific interaction with Pr55(Gag). Our analysis of gRNA fragments of different length supports the existence of a long-range tertiary interaction involving sequences upstream and downstream of the Psi region. This long-range interaction might promote optimal exposure of SL1 for efficient Pr55(Gag) recognition. Altogether, our results shed light on the molecular mechanisms allowing the specific selection of gRNA by Pr55(Gag) among a variety of svRNAs, all harboring SL1 in their first common exon.
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