期刊
REVIEW OF SCIENTIFIC INSTRUMENTS
卷 87, 期 11, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.4967864
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资金
- MRC [MC_U105192715, MC_U105184326] Funding Source: UKRI
- Medical Research Council [MC_U105192715, MC_U105184326] Funding Source: researchfish
- Medical Research Council [MC_U105184326, U105192715, MC_U105192715] Funding Source: Medline
Here we describe a bench-top cryostat system to control the temperature of liquid ethane in a cryo-plunge apparatus designed for biological specimen preparation for electron cryomicroscopy. It comprises a foam insulated Dewar containing a copper cryostat cup, whose temperature is controlled via an active feedback system to within 0.1 K. The device can easily be incorporated into existing manual and semi-automatic cryo-plunge instruments that are not equipped with cryogenic temperature control. Over the course of normal use, we find that using a cryostat is convenient, fast, and does not require special mixtures of cryogens like ethane/propane. This simple cryostat improves the reliability and reproducibility of biological specimen preparation for electron cryomicroscopy. (C) 2016 Author(s).
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