期刊
RESEARCH IN MICROBIOLOGY
卷 167, 期 2, 页码 63-71出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2015.10.001
关键词
Campylobacter jejuni; LC-MS; Chicken colonization; Fatty acid biosynthesis
类别
资金
- Japan Society for the Promotion of Science (JSPS) [26870869]
- Ministry of Health, Labor and Welfare, Japan [H27-shokuhin-ippan-010]
- Grants-in-Aid for Scientific Research [26870869] Funding Source: KAKEN
Campylobacter jejuni is one of the leading causes of foodborne gastrointestinal illness worldwide. Here we performed ex vivo proteomic analysis of C. jejuni 81-176 in chicken, a main reservoir for human infection. At 0, 1 and 4 weeks post-infection (p.i.) with the GFP-expressing 81-176 strain, inocula were recovered from chicken ceca by cell sorting using flow cytometry. iTRAQ-coupled 2D-LC-MS/MS analyses that detected 55 C. jejuni proteins, among which either 3 (FabG, HydB, CJJ81176_0876) or 7 (MscS, CetB, FlhF, PurH, PgIJ, LpxC, Icd) proteins exhibited >1.4-fold-increased expression at 1 or 4 week(s) p.i. compared with those at 0 weeks p.i., respectively. Deletion of the fabG gene clearly decreased the proportion of bacterial unsaturated fatty acids (UFAs) and chicken colonization. The UFA proportion of the parental strain was not altered when grown at 42 C. These findings suggest that FabG might play a pivotal role in UFA production, linked to bacterial adaptation in the poultry host. To our knowledge, this is the first example of ex vivo C. jejuni proteomics, in which fatty acid metabolism might affect bacterial adaptation to the chicken host. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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