期刊
REPRODUCTIVE SCIENCES
卷 24, 期 1, 页码 114-123出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1933719116650755
关键词
methylation; environmental factor; male infertility; smoking
资金
- Program Cai Yuanpei of China Scholarship Council [[2012]6012]
- National Natural Science Foundation of China [81573215, 81273170, 81370083]
- National 12th Five-Year Plan for Science and Technology Support, China [2012BAI37B03]
- Australia NHMRC grant [APP1112767]
- Edith Cowan University-Strategic Research Fund (SRF)
- Importation and Development of High-Calibre Talents Project of Beijing Municipal Institutions [CITTCD201404185]
Male infertility is a complicated disease with causes generally split into 2 broad categories: genetic factors and environmental factors. The present study was designed to investigate the association between the methylation patterns of H19 and SNRPN imprinting control region (ICR) and male infertility and to assess the gene-environment interactions between environmental factors and methylation patterns. A total of 205 DNA samples from 48 oligozoospermia (OZ), 52 asthenozoospermia (AZ), 55 teratozoospermia (TZ) patients, and 50 normozoospermia (NZ) men were analyzed. The mean methylation level of H19-ICR in OZ (80.40% +/- 12.74%) and AZ patients (81.17% +/- 13.18%) was significantly lower than methylation in men with NZ (88.51% +/- 10.54%, P<.001, P<.001, respectively). The mean methylation level of SNRPN-ICR in AZ patients (7.74% +/- 5.71%) and TZ patients (9.33% +/- 5.48%) was significantly higher than in NZ men (6.32% +/- 3.54%, P<.001, P<.001, respectively). Among environmental factors, smoking was correlated with OZ (odds ratio [OR] = 5.12, 95% CI: 2.05-12.83), AZ (OR = 5.65, 95% CI: 2.13-14.99), and TZ (OR = 5.54, 95% CI: 2.21-13.89). Gene-environment interaction analysis revealed that hypomethylation of H19-ICR in OZ patients and hypermethylation of SNRPN-ICR in AZ and TZ patients were significantly associated with an increased the risk of infertility in men who were smokers (OR = 15.30, 95% CI: 1.13-207.97; OR = 13.20, 95% CI: 1.21-143.57; OR = 10.59, 95% CI: 1.04-107.39, respectively). This study demonstrated that hypomethylation of H19-ICR and hypermethylation of SNRPN-ICR are associated with male infertility, and the risk is potentiated by smoking.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据