期刊
RENEWABLE ENERGY
卷 98, 期 -, 页码 72-77出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.renene.2016.02.038
关键词
Cyanobacteria; Biodiesel; FAME; GC-MS; Semi-quantitative RT-PCR
In this study, two freshwater cyanobacteria, Oscillatoria sp. 50A and Synechocystis sp. NN have been evaluated for biodiesel production. Among the two cyanobacteria, Synechocystis sp. NN was isolated, identified by its 16S rRNA gene sequencing. Effects of sodium bicarbonate (SBC), tannery effluent (TE), coir pith (CP) and light stress (L1) on biomass and lipid production of Synechocystis sp. NN were studied. Result showed that maximum biomass productivity of 18.7 +/- 0.9 mg/L/day (1.9 folds) was observed in TE supplemented BG-11 media than normal BG-11 media. Meantime, maximum lipid productivity of 2.6 +/- 0.4 mg/L/day (1.4 folds) was observed in CP supplemented BG-11 media than normal media. Further, fatty acid composition analyses by GC MS showed that C16, C18:1 in Oscillatoria sp. 50A and C16, C20:1, C22:1 in Synechocystis sp. NN were predominant and the fuel properties were also in accordance with the international standards. Besides gene expression of acetyl CoA carboxylase D of Synechocystis sp. NN, analyzed by RT-PCR revealed that transcripts of accD were up-regulated by 1.2-4.7 folds in different media conditions. The findings of this study showed that Synechocystis sp. NN can be utilized as a suitable feedstock that is amenable for cultivation using wastes as nutrient source. (C) 2016 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据