期刊
RENAL FAILURE
卷 38, 期 4, 页码 521-529出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/0886022X.2016.1145521
关键词
stem cells; cisplatin; Acute kidney injury; bone marrow transplantation; bone marrow cells
资金
- Fondation de l'Avenir, Paris, France [ET0-570]
- Region Ile de France (CORDDIM)
- Agence Regionale de Sante (from Ile de France)
- Agence Regionale de Sante (from Amiens, France)
- French National Institute for Health and Medical Research (INSERM)
It has been suggested that bone marrow derived stem cells have the ability to engraft the kidney and improve the outcome of severe acute kidney injury (AKI) in mice exposed to high doses of cisplatin, providing hope for cancer patients in whom irreversible renal damage occasionally occurs following the use of this highly effective anti-tumor drug. We tested the therapeutic potential of bone marrow derived cells injected during the acute phase (day 3 after cisplatin administration) of experimentally-induced AKI in C57Bl6/J mice, characterized by massive tubular necrosis, apoptosis, and a low proliferation capacity. We failed to show any benefit of bone marrow derived cells versus a regular homogenate of intact renal cells, or normal saline. Using cell tracers and flow cytometry, we demonstrated that bone marrow derived cells did indeed home to the bone marrow of the recipients but failed to settle in the kidney. Conversely, renal cells homed to injured kidneys. However, neither cell therapy protected the animals against cisplatin-induced death. We therefore question the short-term efficacy of bone marrow derived cells used to repair established injuries of the tubular epithelium.
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